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导?PCR?DNA?1.?去?模?PCR?221、.?PCR?3.?PCR?36?23.?Synthetic?Biology?Journal?24.?Synthetic?Biology?Journal?37?25.?Synthetic?Biology?Journal?2.4.3?务?研?撑?iBioFoundry?模?38?PCR?研 OD?研?OD?研?撑?研?研?务?务?研?务?研?1.?研?OD?研?2.?研?研?撑?3.?研?研?26.?Synthetic?Biology?Journal?2.4.4 iBioFoundry?1.?iBioFoundry 务?割?务?39?iBioFoundry?DNA?2.?iBioFoundry?务22、?务?iBioFoundry?务?3.?务?iBioFoundry 务?务?40?3?展?-?-?-?封?封?告?务?割?20?割?封?务?割模?去?割?务?封?历?撑练?割?2018?6?15?去?去?封?1.?2.模?撑?3.?的?务?4.?够?封?封?3.1?去?匹?撑练?41?研割?,?封?务?去?研?研?3.2 SDLs?-?SDLs?覆?1.?TRY?Titer?Rate?Yield?务?TRY?去?确?-?-?告?务?2.?去?3.?4.?告?务?5.确?/?导?确?务?SDLs?割?SDL?撑练?SDLs?SDLs?SDLs?SDLs?研?务?SDLs?42?封?撑?匹?研?覆23、?SDLs?27.?SDLs?SDLs?务?SDLs?务?SDLs?SDLs?够?确?SDL1?SDL2?SDLs?务?研?封?撑练?ScienceDirect?43?3.3?SDLs?模?SDLs?覆?研?封?SDLs?务?告?SDLs?诱务?封?导?DNA?去?导?务?研?44?4?,?,?,?,?&?.?(2023).?.?,?39(2),?11.?,?,?,?,?,?&?.?(2021).?.?,?37(3),?37.?.?(2018).?:?.?,33(11),?1135-1149.?.?(2022).?:?.?,?38(11),?11.?,?,?,?,?,?&?.?(2021).?24、.?,?2(6),?13.?,?.?(2023).?.?.?(2022).?.?,?,?,?,?.?(2022).?.?,?,?,?,?.?(2022).?.?2022?-?-?4Q?2021?Synthetic?Biology?Venture?Investment?Report?Synbiobeta?,?,?&?.?ibiofoundry?.?,?1-14.?Jinming,?C.?,?Bingzhao,?Z.?,?Yingfei,?M.?A.?,?Xiongfei,?F.?U.?,?Meng,?W.?,?&?Chenli,?L.?.?(2018).?Engineering?platform25、s?for?synthetic?biology?research.?(11).?Yu,?T.,?Boob,?A.?G.,?Singh,?N.,?Su,?Y.,?&?Zhao,?H.?(2023).?In?vitro?continuous?protein?evolution?empowered?by?machine?learning?and?automation.?Cell?systems,?S2405-4712(23)00115-1.?Advance?online?publication.?Martin,?H.?G.,?Radivojevic,?T.,?Zucker,?J.,?Bouchard26、,?K.,?Sustarich,?J.,?Peisert,?S.,?Arnold,?D.,?Hillson,?N.,?Babnigg,?G.,?Marti,?J.?M.,?Mungall,?C.?J.,?Beckham,?G.?T.,?Waldburger,?L.,?Carothers,?J.,?Sundaram,?S.,?Agarwal,?D.,?Simmons,?B.?A.,?Backman,?T.,?Banerjee,?D.,?Tanjore,?D.,?Singh,?A.?(2023).?Perspectives?for?self-driving?labs?in?synthetic?bi27、ology.?Current?opinion?in?biotechnology,?79,?102881.?Gurdo,?N.,?Volke,?D.?C.,?McCloskey,?D.,?&?Nikel,?P.?I.?(2023).?Automating?the?design-build-test-learn?cycle?towards?next-generation?bacterial?cell?factories.?New?biotechnology,?74,?1?15.?Carbonell,?P.,?Radivojevic,?T.,?&?Garca?Martn,?H.?(2019).?Op28、portunities?at?the?Intersection?of?Synthetic?Biology,?Machine?Learning,?and?Automation.?ACS?synthetic?biology,?8(7),?1474?1477.?HamediRad,?M.,?Chao,?R.,?Weisberg,?S.,?Lian,?J.,?Sinha,?S.,?&?Zhao,?H.?(2019).?Towards?a?fully?automated?algorithm?driven?platform?for?biosystems?design.?Nature?communicati29、ons,?10(1),?5150.?Chao,?R.,?Liang,?J.,?Tasan,?I.,?Si,?T.,?Ju,?L.,?&?Zhao,?H.?(2017).?Fully?Automated?One-Step?Synthesis?of?Single-Transcript?TALEN?Pairs?Using?a?Biological?Foundry.?ACS?synthetic?biology,?6(4),?678?685.?Liu,?L.,?Huang,?Y.,?&?Wang,?H.?H.?(2023).?Fast?and?efficient?template-mediated?sy30、nthesis?of?genetic?variants.?Nature?methods,?20(6),?841?848.?Enghiad,?B.,?Xue,?P.,?Singh,?N.,?Boob,?A.?G.,?Shi,?C.,?Petrov,?V.?A.,?Liu,?R.,?Peri,?S.?S.,?Lane,?S.?T.,?Gaither,?E.?D.,?&?Zhao,?H.?(2022).?PlasmidMaker?is?a?versatile,?automated,?and?high?throughput?end-to-end?platform?for?plasmid?construction.?Nature?communications,?13(1),?2697.?Smanski,?M.?J.,?Mead,?D.,?Gustafsson,?C.,?&?Thomas,?M.?G.?(2017).?Meeting?Report?for?Synthetic?Biology?for?Natural?Products?2017:?The?Interface?of?(Meta)Genomics,?Machine?Learning,?and?Natural?Product?Discovery.?ACS?synthetic?biology,?6(5),?737?743.?